A large number of medicinal plants are claimed to be useful in skin disease in all traditional system of medicine. Plumeria rubra is a deciduous tree, widely distributed in common rather moist garden and in lawns. Plant loses leaves for a short time during the winter. Slowly grows up to 25 feet. Plumeria rubra is nowadays, is gaining interest by the scientific researchers due to its folklorics and because of its therapeutic value, easy availability. The result showed that a ethanolic extract exhibited a significant activity with specific standard (chloromphenicol) and antifungal activity with specific standard (Fucanazole). Hence this study proves that Plumeria rubra possess antimicrobial activity.