American Journal of PharmTech Research AJPTR 2249-3387 undefined AJPTR52016 RP-HPLC Method development and validation for the Estimation of Etoposide in Bulk Drug and its Pharmaceutical Dosage Form. B.Lakshmi B.Lakshmi Krishna K.Rama K.N.Jayaveera K.N.Jayaveera rao G.V.Padmakar Department of Chemistry, GITAM University, Hyderabad- 502329, INDIA Department of Chemistry, GIS, GITAM University, Visakhapatnam – 530045, AP, INDIA Department of Chemistry, JNTU, Anantapur- 515002, AP, INDIA Aurobindo Research Centre, Hyderabad-500090, India. 04 01 2015 5 2

A specific and accurate HPLC method is developed for the determination of etoposidein bulk drugs and in solid capsule dosage form. Best symmetric peak shape obtained with Inertsil ODS C-18 column (250 X 4.6 mm, 5μ) column in an isocratic mode, with retention time 5 min.The mobile phase used was Water : Acetonitrile  60:40(v/v)with flow rate 1.0 ml/min and effluent was monitored at 263 nm. As per ICH guidelines method has validated. Method has found linear in the range of 5-45 µg/ml. The LOD and LOQ were found to be 0.02 and 0.06 µg/ml respectively. Method was found specific with respective of diluents, excipients and degradants.

 Etoposide RP-HPLC ICH guidelines Method validation.