Hydrotropicsolvents may proper choice to preclude the use of organic solvents so that, a simple, accurate, novel, safe and precise method could developed for estimation of poorly water soluble drug,. Solubility of Daurnavirincreased by using 8M urea as a hydrotropic agent. Daurnavir1showed the maximum absorbance at 263.91nm in method A,254-274 nm in method B and 264nm in method C. At these wavelengths, hydrotropic agent and other tablet excipients did not show any significant interference in the spectrophotometric assay. The developed methods were found to be linear in the range of5-40 µg/ml for method A, method B&C with correlation coefficients (R) of 0.997, 0.995 and 0.997 respectively.The mean percent label claim of tablets of of Daurnavir in formulation estimated by the proposed methods was found to be107%. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical parameters were found to be good accordance with the prescribe values. As hydrotropic agent was used in the proposed methods, these methods were eco-friendly and it can be used in routine quantitative analysis of drug in bulk and dosage form in industries. Key words:Daurnavir, urea;  AUC; Hydrotropicsolubilization technique; derivative spectroscopy.