Anthocyanins are multifunctional bioactive compounds implicated with beneficial health effects due to their strong antioxidant activity for scavenging free radicals that are involved in cell damage and tumour promotion. Evidence has accumulated to suggest that flavonoids potently suppress tumour cell proliferation and may effectively work as chemopreventive agents against carcinogenesis in humans. In the study, we detected the antiproliferative effects of anthocyanin on human epithelial cells and the Breast cancer cells by MTT assay. The HEp-2 and MCF-7 cells were seeded in 96-well culture plates in different concentrations of cassava leaf stalk extracts of Indian Cassava (Manihot esculenta, Crantz ) to determine their anticancer effects using the MTT assay. Anthocyanin extracted from cassava leaf stalk extracts have 79% inhibition on HEp-2cells at 1000 μg/ml (Table1). The anthocyanin extracted from cassava leaf extracts was tested against MCF-7 cell lines shows 78% inhibition at 1000 μg/ml (Table 2). So we confirm that, the anthocyanin compounds present in cassava leaf extracts are inhibiting the proliferation of the cancer cells.