Poisonous plants may be a threat to the health and safety of people and animals. It makes crucial to have knowledge about surrounding poisonous plants. Information gathered by generating database of poisonous materials from any particular region may be life saving. Chemical analysis of these plants is another important aspect especially for forensic experts solving crime cases involving poisonous plants. Therefore this paper has also conducted Thin Layer Chromatographic separation of poisonous plants. A wide diversity of plants could be seen in and around Rohtak city of Haryana but literature survey didn’t reveal any significant information about the poisonous plants from this area. Therefore, the present study has inspected occurrence of some poisonous plants throughout Rohtak city and their chromatographic analysis. Importantly the results have been of great utility. Morphological examination identified ten poisonous plants vis. Thevetia peruviana, Nerium odoratum, Calotropis procera, Ricinus communis, Datura fastusa, Argemone mexicana, Ipomea marginate, Crinium asiaticum, Plumeria obtuse and Abrus precatorius from different localities of the selected area.  A new single solvent system chromatographically separating all these poisonous plant extracts has also been developed.