Phytochemical are compounds derived from natural source play important role in mankind health system with various physiological activities such as antioxidant and thrombolytic activity. Borago officinalis leaves have been assessed for quantity of antioxidant compounds comprise in estimation of total phenolic, total flavonoid and total tannin contents, and thrombolytic activity of hydrolacholic leaves extract at different concentration ranges. The hydroalcholic extract of leaves showed low phenolic contents about (26.45 ± 0.126 mcg GAE\g DW) with approximately similar quantity of total flavonoid and total tannin contents about (212 ± 0.278 mcg QE\g DW) and (216 ± 0.521 mcg GAE\g DW), respectively. Leaf extract exhibited a dose dependent manner thrombolytic activity ranges between (8.346± 0.669 - 44.6697±1.1076) considered significant in comparison with control negative distilled water (p value < 0.0001) and moderate activity in comparison with control positive streptokinase. Borago officinalis leaf was a good source for antioxidant compounds and open a venue for production of thrombolytic agents from the leaf with less side effects in comparison to the conventional drugs.