Life co-existed with disease, decay and death. The study of disease and their treatment is related with the human intellect. Illness has been the man’s heritage from the beginning of his existence and the search for remedies to combat the diseases is perhaps equally old. Melghat region is a rich source of medicinal flora. Cassineglauca is one of the medicinally potential plants found in Melghat region which is frequently used for the eradication of skin diseases1. The present investigation was undertaken to screen the efficacy of Cassineglauca whole extract, isolated acidic ingredient and its newly synthesized analogues on certain bacteria which are responsible for skin diseases such as Streptococcus pyogenes, Nocardia calcarea, Bacillus subtilis, and Pseudo monasaeruginosa. In comparison among the impact of test compounds; it was revealed that, the Cassine glauca isolated acidic ingredient and its newly synthesized analogues showed significant activity than the whole extract.