Two simple, precise and economical UV methods have been developed for estimation of Methotrexate in bulk formulation. Method A involves measurement of UV absorbance in Zeroorder derivative at 303nm. Method B deals with area under curve measurement (AUC method), which involves the calculation of integrated value of absorbance with respect to wavelength between 300-306nm.  The drug follows Beer-Lambert’s law in the concentration range of 3-15μg/ml in both the methods. Results of analysis were validated statistically and were found to be satisfactory. Thus proposed methods can be successfully applied for estimation of Methotrexatein routine analytical work.