Gemifloxacin mesylate loaded microspheres were prepared by Ionotropic gelation technique with different drug to carrier ratio. All the microspheres were characterized for particle size, scanning electron microscopy, FTIR study, DSC, percentage yield, drug entrapment, stability studies and for in vitro release kinetics and found to be within the limits. Among all the formulations S8, was selected as optimized formulation based on the physic chemical and release studies. In the in vitro release study of formulation S8 showed 95.92%, after 12 h in a controlled manner, which is essential for anti ulcer therapy. The innovator Gemiflox conventional tablet shows the drug release of 95.23% within 1 h. The drug release of optimized formulation S8 followed zero order and Higuchi kinetics indicating diffusion controlled drug release.