A simple, rapid, sensitive reverse-phase high-performance liquid chromatography method was developed and validated for simultaneous estimation of Cefixime and linezolid ,at single wavelength of 263nm.chromotographic separation was performed on an enable aligent zorabax(thermo) column(250nmx4.6mm ID particle size 5 um) and a  mobile phase consisting of acetonitrile and water(70:30v/v) at a flow rate of 1.0ml/min. the calibration curve was linear(r2≥0.0999) over the concentration range. 400-1200μg/mL of cefixime and 1200-3600μg/mL of linezolid. the limit of quantification was 9.709µg/ml for Cefixime and  9.482µg/ml  for linezolid no interference was found by the excipients in the synthetic mixture. The proposed methods were validated for international conference on harmonization guidelines for linearity, accuracy, precision, and robustness for estimation of Cefixime and linezolid in bulk and synthetic mixture, and the results were found to be satisfactory