Leaves of Barleria prionitis (family- Acanthaceae) are used in traditional medicines for various ailments. The present study is to investigate the antipyretic activity of methanolic extract of Barleria prionitis in Albino rats. Brewer’s yeast induced pyrexia method was selected for evaluation. The standard drug used was paracetamol (150 mg/kg/p.o). Neck and Rectal temperature were recorded by digital thermometer and compared with the control group. Based on the results obtained, the methanolic leaf extract of Barleria prionitis showed significant antipyretic activity.