Spermacoce hispida possesses significant anti-oxidant and cardioprotective activities. This manuscript reveals the preventive potential of seed extract on lysosomal damage in isoproterenol induced myocardial infarction (MI) in rats. Two different doses of the plant extract such as 100 and 200 mg/kg body weight was used to prove the cardioprotective effect against 100mg/kg body weight of isoproterenol (ISO). The activities of lysosomal enzymes in the heart of ISO- induced MI rats were increased significantly.  Pretreatment with hydro alcoholic extract of seeds of Spermacoce hispida prevented the changes in the levels of lysosomal enzymes in isoproterenol induced MI rats. The activity of enzymes like β –D- Galactosidase, β –D- Glucuronidase, N-Acetyl β-D –glucoseaminidase, Cathepsin D and ATP ases are found to be increased in heart homogenate in diseased rats against normal animals.