The rapid spread of multidrug-resistance against conventional antibiotics is a global threat that necessitates the search for alternative therapies from natural sources. In this investigation, the antibacterial potentials of ethanol extracts of moth beam (Vigna aconitifolia)  extracts were evaluated against Bacillus subtilis, Staphylococcus aureus, Staphylococcus werneri, Pseudomonas aeruginosa and Escherichia coli, Proteus mirabilis, Kleibsella pneumonia, Pseudomonas putida, using Agar cup-plate method. The extract showed  antimicrobial  activities  against  all  the  tested  gram positive and  gram negative  bacteria. Ethanol extracts in general exhibited greater antibacterial activity and results were comparable to the standard. Our finding identifies the potential use of moth bean as a natural source of antibacterial agent. Further studies are warranted to elucidate the mechanism of action of the extracts and to identify the active components responsible for the antibacterial activity.