The antiulcer effect of cow urine extract of Piper betel (family Piperaceae) was evaluated in pyloric ligation induced ulceration model in Wistar rats. The antiulcer activity was evaluated by measuring ulcer index and percentage of ulcer healing. The extract (500mg/kg b.w) showed significant antiulcer activity as evidenced from the data obtained. Histopathological findings also confirm the antiulcer activity of Cow urine Piper betel extract.