This study was performed to evaluate the antimicrobial activity of Hibiscus vitifolius. The ethyl acetate fraction of Hibiscus vitifolius were shown to possess an antimicrobial activity against bacteria and fungi, viz. Six bacterial strains were S. typhi, E. coli, E. faecalis, B. cereus, B.Substilis, Lacto bacillus and two fungal strains C.lunata, C.albicans  by using disc diffusion method. The anti bacterial activity of ethyl acetate fraction is almost comparable with standard solvent control Chloramphenicol. The anti fungal activity of ethyl acetate fraction extract is almost comparable with standard solvent control Fluconazole. Further studies are highly needed for future drug development.