A novel complex of quadridentate ligand modified chitosan was synthesized by sequential attachment of Isatin (1H-indole-2, 3-Dione) copper chloride dehydrate to chitosan. Synthesized catalyst was characterized by different techniques like FTIR, ESI-MS, and Powder X-ray diffraction method. The biological activity was done using diffusion disc technique. It was found that the complex shows excellent activity towards fungi and bacteria. The catalytic activity of this catalyst was tested for the oxidation of 2-butanol by varying the temperature of system as well as concentration of substrate and catalyst. Values of activation energy have been evaluated from the kinetic data.