Thiourea and urea are important functional groups in various natural products and drug intermediates. They are used as neutral receptor for various anions (anion complexation) and building blocks for various heterocycles. Sugar-based thioureas are a new class of molecules promising for many biological applications. Here, we have synthesized a new range of 1-β-D-glycosyl-3-aryl thiocarbamides by the interaction of various glycosylamines with aryl isothiocyanates. The structure of title compounds were confirmed by their IR, NMR mass spectral data and elemental analysis. The newly synthesized compounds were screened for their in vitro antimicrobial activities using standard cup plate method against bacteria E. coli, P. aeruginosa, S. aureus and fungi A. niger, T. viride. The title compounds exhibited mild to moderate antimicrobial activities.