Plants play many important roles in the environment. Not only do they provide us with food and useful products, but also they play a main role in maintaining the ecological balance of nature, the food chain and the natural cycles and also have medicinal value. Laboratory evaluations were made to assess the study of primary metabolites of Tinospora cordifolia, belongs to the family menispemeacae. It contains chlorophyll, sugars, starch, protein, ascorbic acid and phenols in, leaves, stem, root and callus of the plant. Levels of plant metabolites are strongly affected via genetic and environmental factors. Different types of pests’ are causing changes in plant metabolite production. The results revealed the evidence of different infestation of the plant by common herbivores. In this manuscript we report primary metabolites of the Tinospora cordifolia along with the quantification after the pests’ effect.