American Journal of PharmTech Research AJPTR 2249-3387 undefined AJPTR66031 UV Visible Spectrophotometric Estimation of Antibiotic Drugs Reddy C.M.Bhaskar Reddy Dr G.V. Subba Reddy Dr N.Ananda Kumar Research Scholar ,Dept of chemistry ,Rayalaseema University ,Kurnool ,AP, India. Jawaharlal Nehru Technological University Anantapur College of Engineering (Autonomous),Pulivendula,Kadapa (dist),AP , India 516 390 12 01 2016 6 6

The main aim of current study was to develop U V visible spectrophotometric method for the quantitative determination of Flucloxacillin and  Ceftriaxone in bulk and  pharmaceutical formulations. Flucloxacillin  is a  broad spectrum beta -lactam antibiotic, belonging to the isoxazolyl family of penicillin’s. Ceftriaxone is a semi synthetic, broad-spectrum and third generation  cephalosporin antibiotic for intravenous or intramuscular administration. The solvents used in this method is chloroform ,double distilled water (1:1) in presence of phosphate buffer of pH 7.4. Linearity test solutions for the assay method were prepared in the range of 2-12 μg/ mL and  4-16 μg/mL respectively for determination of flucloxacillin and ceftriaxone which obeyed Beer’s law and found to linear. From the experimental results reveal that absorption maximum of 270 nm for flucloxacillin and 290 nm for ceftriaxone were found. Validation of the developed method for two drugs was carried out as per ICH requirements.

 Flucloxacillin ceftrixone U v visible spectrophotometry Beer’s law validation.