A new, simple and sensitive UV-spectrophotometric method was developed for the determination of Benfotiamine in bulk and tablet dosage form. This method, involves the measurement of absorbances of Benfotiamine at the wavelength of 244nm. 0.1M HCl was used as solvent. Linearity was observed in the concentration range of 3-18µg/ml with correlation coefficient 0.999. The accuracy of the method was confirmed by recovery studies of tablet dosage forms and was found to be 99.32%-100.42% for Benfotiamine. The method showed good reproducibility and recovery with %RSD lessthan 2.0. The LOD and LOQ of Benfotiamine was found to be 0.051μg/ml and 0.155μg/ml. Results of the analysis were validated for accuracy, precision, LOD, LOQ and were found to be satisfactory. Thus the developed method was found to be simple, sensitive, rapid, precise, accurate and cost effective quality control tool for the routine analysis of Benfotiamine in bulk and tablet dosage form.