Isoamyl-2-cyanoacrylate is a non-toxic, bio-compatible, haemostatic and bacteria static monomeric component in nature. But in presence of moisture it gets converted into polymer. It is used as tissue Bio-adhesive; it is an alternative to suturing in medical and cosmetic surgeries. It is an inert material solidifies rapidly in alkaline media and slowly solidifies in acidic media within 5-10 seconds. It has a tendency of rapid wound closure with minimal scarring and reduces the subsequent infection. It is less traumatic and more efficient, precise and safe. It effectively replaces the conventional wound closure procedures. It is an active component in brand name “Amcrylate” is manufactured and promoted by” Concord drugs Limited” Hyderabad. The analytical gas chromatographic method is developed for the estimation of purity of isoamyl-2-cyanoacrylate, procedure as follows.