The present study was undertaken to identify the active principles in leaf, Stem and Root extracts of Ruta graveolens L. using gas chromatography-mass spectrometry (GC-MS). The plant material was extracted in methanol by using Soxhlet apparatus for 24 hours and the extracts were analyzed using GC-MS. The mass spectra of the compounds observed were matched with the National Institute of Standards and Technology (NIST) library. The results revealed the presence of 17, 12 and 6 chemical compounds in leaf, stem and root extracts respectively. The major chemical constituents were kokusaginine (39.9%), Bergaptene (25%),2-Undecanone (14.97%),1-(1,3-Benzodioxol-5-ylmethyl)-3-Nitro-1(12.69%),Pyrrole-3-carboxaldehyde,1-(4- methoxyph(9.36%).Some of these compounds have been reported to possess various bioactivities such as anti-microbial, anti-viral, anti-oxidative, anti-proliferative, anti-inflammatory, anti-tumour,etc. The study is a base to understand the richness of bioactive components in methanolic extracts from different parts of Ruta graveolens L. Their isolation would have potential applications in drug designing as well as in the medicine and healthcare industries.