Some novel series of pyrazoline derivatives were synthesized from Chalcones. Chalcones were prepared by treatment of Paracetamol with different aldehyde by Claisen-Schimidt Condensation. Various Pyrazoline derivatives were prepared by reflux reaction of Chalcone with Phenyl Hydrazine/Hydrazine Hydrate in ethanolic solution. The structures of the newly synthesized Pyrazoline derivatives have been established on the basis of their spectral data. Structures of compounds were confirmed by IR, H1 NMR. The synthesized selected compounds were screened for their antimicrobial activity.