The main objective of the present investigation is to evaluate the analgesic activity of hydro-alcoholic extract of Acacia Senegal pod on mice. Analgesic activity of hydro-alcoholic extract of Acacia Senegal pod at a dose of 200 mg/Kg, 400 mg /Kg and 800 mg /Kg were evaluated against drug pentazocine at a dose of 17.5 mg/Kg. Adult albino mice and rats of either sex of six numbers in each group were under taken for study and evaluated by eddy’s hot plate and tail immersion method. The all doses of Acacia Senegal pod crude hydro-alcoholic extract were found to produce significant (P