A variety of novel clubbed triazolyl indeno[1,2-c]isoquinolines 14-21 derivatives were synthesized in good yields and characterized by IR, 1H NMR, mass spectral and elemental analyses. All the newly synthesized compounds were evaluated for their in-vitro anticancer activity and topoisomerase I inhibition. Several compounds showed interesting cytotoxic activities when compared with the Doxorubicin as a reference drug.