This study was designed to evaluate, aqueous and ethanol leaf extracts of Barleria longiflora L.f. for anti-inflammatory activity using Formalin induced paw oedema in rats. Animals were divided into 6 groups of 6 rats. Groups 1 and 2 served as Formalin induced control and standard (Diclofenac 5 mg/Kg, i.p.) respectively. Groups 3 and 4 were treated, orally with aqueous extract of Barleria longiflora L.f. of 100 and 400 mg/kg b.w, respectively. Groups 5 and 6 were administered, orally with ethanol extract of Barleria longiflora L.f. of 100 and 400 mg/kg b.w, respectively. The paw volume was measured at 0, 1, 2, 3 and 4 hr after Formalin injection. The actual edema volume was calculated. The data was expressed as mean ± S.E.M. The statistical analysis was done by means of ANOVA followed by Dunnett’s post hock test. The aqueous and ethanol leaf extracts of Barleria longiflora L.f. exhibited significant reduction of edema in the rats. Ethanol leaf extract of Barleria longiflora L.f. revealed surpassing anti-inflammatory activity.