The aim of present study was to assess the anti-inflammatory activity of polyherbal formulation of leaves of H. aciatica, C. gigantean & A. aspera were collected and authenticated. Extractions of dried leaves and rhizome were carried out with ethanol in soxhlet apparatus. The polyherbal formulation showed the significant anti-inflammatory activity comparable to the standard drug Diclofenac sodium against carrageenan induced rat paw edema method. The polyherbal formulation reduced the inflammation induced by carrageenan by 49.3% and 61.73% on oral administration at 100 mg/ kg and 200 mg/kg respectively as compared to the control treated group.