A fluconazole o/w microemulsion was developed for topical application using isopropyl myristate as the oil phase. Pseudo-ternary phase diagrams were constructed for the determination of existence region of micro emulsion region using the surfactant (tween 80 & Cremophor RH-40) and co-surfactant (ethanol). Different formulations were prepared for the evaluation of oil content, surfactant/co-surfactant concentration on in-vitro permeation rates. In-vitro transdermal permeability of flucanazole from the micro emulsions was evaluated using Keshary Chien diffusion cells mounted with 0.45µ with cellulose acetate membrane. The amount of drug (Fluconazole) permeated was analyzed by HPLC.