The present study was carried out for screening of preliminary phyto-chemical constituents and anti-microbial activity of ethanolic extract of ASCLEPIAS CURASSAVICA. The dried extract obtained by soxhlet extraction was subjected to qualitative phyto-chemical screening for the identification. The anti-microbial activity of A.curassavica flower & plant extract by agar well diffusion method. Our study reveals that B.subtilis & E.coli showed good inhibitory zone in ethanol solvent extract