A study has been done to examine the indicator action of methanolic extract of flower Calotropis gigantean Linn and development of litmus paper. Calotropis gigantean Linn belongs to family Apocynaceae, Methanolic extract of flower Calotropis gigantean Linn was examined and compared with that of previously present synthetic indicators. Flowers were extracted using methanol, a specific volume was added which gave perfect and reliable results for all the four different types of neutralization titrations. Developed litmus paper shows changed colour in Basic medium. The work shows that natural indicator and developed litmus paper was very useful, economical, simple and accurate.