Adult mammals undergo minimal regeneration having cardiac injury or ongoing burden of heart failure. Recent studies have established the importance of several evolutionary conserved mechanism of heart renovation by cyclic division of differentiated cardiomyocyte, cardiomyocyte proliferation, angiogenesis, extracellular matrix and heart regeneration, tissue engineering, transcription profile of cardiomyocytein development and regeneration, stem cell derived cardiomyocyte, and there transplantation. The c-kit-expressing cardiomyocyte stem cell also provide. The DNA translation is also occurs in regeneration process. In the review is part of special issue related to continuous renovation on cardiomyocyte and there related mechanism involved and gives future concept about novel biotechnology and tissue engineering.