Alogliptin Benzoate is a novel hypoglycemic drug that belongs to dipeptidylpeptidase-4 inhibitor class which stimulates glucose dependent insulin release. The Present work describes development and validation of a new simple, accurate, precise and stability indicaing HPTLC method for the determination of alogliptin benzoate in tablet dosage form. The chromatographic separation was achieved by using Chloroform: Methanol 3:7 v/v as mobile phase and UV detection at 275 nm. The developed method was validated with respect to linearity, accuracy, precision, limit of detection, limit of quantitation and robustness as per ICH guidelines. The drug was subjected to stress condition of acid hydrolysis, alkali hydrolysis, photolysis, thermal degradation. Results found to be linear in concentration range of 500-2500 ng/band. The developed method can be used for the quantification of bulk drug as well as tablet dosage form