Tuberculosis is a ubiquitous, highly contagious chronic granulamatous communicable bacterial infectious disease caused by Mycobacterium tuberculosis and other species of same genera. “Rifabutin” which is useful in the management of tuberculosis. Formulated Rifabutin in the form of solid lipid nanoparticle evaluated for their efficacy ex-vivo by checking for various interactions. Thus, physiological parameters like cellular uptake, MTT cytotoxicity assay and hemolytic toxicity of rifabutin  loaded mannosylated solid lipid nanoparticles are determined and compared. The present work establishes the suitability of rifabutin loaded mannosylated solid lipid nanoparticles as a delivery system.