New Validated HPLC Method for the Estimation of Oxazepam In Pharmaceutical Formulation

A simple, selective, linear, precise and accurate HPLC method was developed and validated for rapid assay of Oxazepam in Bulk and Pharmaceutical tablet Formulation. Isocratic elution at a flow rate of 1.0 ml/min was employed.  The chromatographic analysis was performed on a ODS 5 μm (4.6 mm x 15 cm) or equivalent column at ambient temperature. The mobile phase consisted of Methanol: Water in the ratio of 65:35v/v. The UV detection wavelength was 230nm and 100µl sample was injected. Flow rate was found to be 1.0.  The retention time for Oxazepam was identified. The Average percentage recovery of the method was in the range of 0.5.  The method was validated as per the ICH guidelines. The method was successfully applied for routine quality control analysis of pharmaceutical formulation.