Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Ibuprofen, Paracetamol and Caffeine in Pharmaceutical Dosage Form

A simple, sensitive, accurate and precise simultaneous UV spectrophotometric method has been developed for the estimation of Ibuprofen, Paracetamol and Caffeine in tablet dosage form. The absorption maxima of the drugs were found to be 223, 248 and 272 nm for Ibuprofen, Paracetamol and Caffeine respectively, in methanol, using a Shimadzu UV–Visible spectrophotometer (model UV-1800). Ibuprofen, Paracetamol and Caffeine obeyed Beer’s law in the concentration range of 10-70 µg ml-1, 10-60 µg ml-1 and 10-70 µg ml-1 respectively. The correlation coefficient was found to be 0.999, 0.999, and 0.999 for Ibuprofen, Paracetamol and Caffeine respectively. The method was validated for various parameters according to ICH guidelines. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. Assay results were in good agreement with label claim.