Simultaneous Determination of Lamivudine, Zidovudine and Nevirapine in Tablet Dosage Forms by RP-HPLC

An accurate, precise and economic reversed phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for the estimation of lamivudine, zidovudine and nevirapine in pharmaceutical dosage forms. In this method Qualisil BDS C8 column (250mmx4.6mm i.d., 5µm particle size) with mobile phase containing water and acetonitrile in the ratio of 70: 30 v/v with pH adjusted to 5 with ortho phosphoric acid (OPA). The flow rate was 1mL/min and the detection wavelength was 250nm. The linearity was observed in the range of 1-15µg/mL for lamivudine, 3-24 µg/mL for zidovudine and 2.5-20 µg/mL for nevirapine. Retention times were 3.1min, 4.4min, and 7.0min for lamivudine, zidovudine and nevirapine respectively. The proposed method was validated as per ICH guidelines for linearity, accuracy, precision and robustness and can be applied for routine quality control analysis of pharmaceutical dosage forms used for multidrug therapy containing lamivudine, zidovudine and nevirapine.