Comparison and Quantification of Marker Compound of Triphala Guggulu by using HPTLC method

Triphala guggulu contain the amala,baheda,and harde ,pipali, and guggulu in powder form which contain many phytoconstituents other than phenols(gallic acid,ellagic acid) and alkaloids(piperine) and Z-guggulusterone. phenolics are present in good amounts, and several as important anti-oxidant. Piperine is a bioavailability enhancer and guggulusterone as anticholesterimic. Looking into the importance of these ingredients, attempt has been made for simultaneous estimation of piperine, gallic acid, ellagic acid by using HPTLC methods  A simple HPTLC method has been developed for the estimation of galic acid, ellegic acid,piperine &z-gugggulu sterone from methanolic extract of marketed & lab preparation of triphala guggulu these compound in the extracts has been estimated by using HPTLC method the separation was performed on TLC Aluminum Plates precoated with silica gel 60F254,good separation was achieved in the mobile phase of toluene: ethyl acetate :formic acid: methanol(3:3:0.8:0.5)and densiometric determination of galic acid, ellegic acid carried out at 280 nm and piperine was carried out at 337nm&Z-guggulusterone was carried out at 248 nm & compare the these phytoconstituents with other marketed product