Development and Validation of A Reverse Phase HPLC Method for Determination of Memantine In Pure and Pharmaceutical Formulations

A simple, accurate and precise HPLC method for the estimation of memantine in bulk and pharmaceutical dosage form has been reported. Chromatography was performed with Shimadzu HPLC equipment comprising an LC-10A VP quaternary pump, a variable-wavelength programmable UV–visible detector, an SPD-10AVP column oven, and an SCL 10AVP system controller. A Rheodyne injector fitted with a 20μL loop was also used and data were recorded and evaluated using Class-VP 5.032 software. The Compound was separated, at ambient temperature (25 ± 2°C), on a  BDS C18, ( 4.6 mm i.d x 250 mm, 5μm reversed phase column with 100% methanol as mobile phase at a flow rate of 1.0mL.min−1. Before use, the mobile phase was filtered through a 0.22-μm Nylon filter. UV detection was performed at 274nm. A linear response was observed in the concentration ranges of 5-25μg/ml with a regression coefficient of 0.9999. The method was then validated for different parameters as per the ICH guidelines. This method can be used for the determination of memantine in quality control of formulation without interference of the excipients.