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American Journal of PharmTech Research

American Journal of PharmTech Research

Published

Antibacterial potential of traditional chewing stick plants Mezoneuron benthamianum and Strychnos afzelii against cariogenic Streptococcus spp. in Côte d’Ivoire

Published in October 2026 Issue 5 (Vol. 16, Issue 5, 2026)

Antibacterial potential of traditional chewing stick plants Mezoneuron benthamianum and Strychnos afzelii against cariogenic Streptococcus spp. in Côte d’Ivoire - Issue cover

Abstract

Dental caries is one of the most prevalent oral diseases worldwide and is closely associated with cariogenic microorganisms, particularly bacteria belonging to the genus Streptococcus. In western Côte d'Ivoire, several medicinal plants are traditionally used as chewing sticks for oral hygiene, although their antibacterial properties remain poorly documented. This study evaluated the antibacterial activity of aqueous and hydro-ethanolic extracts of Mezoneuron benthamianum Baill. and Strychnos afzelii Gilg. against Streptococcus spp. isolated from dental caries. Plant extracts were prepared by maceration in distilled water and 70% ethanol. Antibacterial activity was evaluated using the agar well diffusion method, while the minimum inhibitory concentration and minimum bactericidal concentration were determined using the broth macrodilution method. Hydro-ethanolic extraction produced the highest yields, reaching 17.60% for Mezoneuron benthamianum and 16.90% for Strychnos afzelii. Neither the aqueous nor the hydro-ethanolic extracts of Strychnos afzelii exhibited antibacterial activity against the tested strains. In contrast, both extracts of Mezoneuron benthamianum inhibited the growth of all tested Streptococcus spp., with inhibition zones ranging from 13.00 ± 0.50 mm to 18.00 ± 0.58 mm. The minimum inhibitory concentration and minimum bactericidal concentration were both 25 mg/mL, resulting in a minimum bactericidal concentration to minimum inhibitory concentration ratio of 1, indicating a bactericidal effect. These findings provide experimental support for the traditional use of M. benthamianum as a chewing stick plant and suggest its potential source of antibacterial compounds for future oral health applications.

References

  1. [1]World Health Organization. Global oral health status report: towards universal health coverage for oral health by 2030. Geneva: World Health Organization; 2022. Othman L, Sleiman A, Abdel-Massih RM. Antimicrobial activity of polyphenols and their potential application in drug discovery. Molecules 2019; 24(11): 2117. Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med 2018; 13:20. Azwanida NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015; 4: 196. Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol 1998; 60(1): 1-8. Setyaningsih EP, Bahri S, Nurjanah R. Antibacterial activity of ethanol, ethyl acetate, and n-hexane extracts of Strychnos lucida R.Br. against Staphylococcus aureus and Escherichia coli. Jurnal Jamu Indones 2024; 9(2). MBAE: aqueous extract of Mezoneuron benthamianum; SAHE: hydro-ethanolic extract of Strychnos afzelii; SAAE: aqueous extract of Strychnos afzelii. SAAE: aqueous extract of Strychnos afzelii;SAHE: hydro-ethanolic extract of Strychnos afzelii ; MBAE: aqueous extract of Mezoneuron benthamianum; MBHE: hydro-ethanolic extract of Mezoneuron benthamianum. Values represent mean ± standard deviation of three independent experiments. MIC: Minimum inhibitory concentration; MBC: Minimum bactericidal concentration; MBAE: Aqueous extract of Mezoneuron benthamianum; MBHE: Hydro-ethanolic extract of Mezoneuron benthamianum. MIC and MBC were determined only for the active extracts of M. benthamianum. An MBC/MIC ratio ≤4 indicates bactericidal activity, whereas a ratio >4 indicates bacteriostatic activity.

Authors (3)

Ebalah Delphine MONYN Epse KOUAME

Department of Agronomic, Fores...

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Marie Rosine ATSAIN Epse ALLANGBA

Department of Fundamental and ...

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N’Guessan Brigitte KOUAME

Department of Agronomic, Fores...

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AJPTR5160001

AJPTR-02-002485

1-10

2026-10-01

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How to Cite

Delphine MONYN Epse KOUAME, E., Rosine ATSAIN Epse ALLANGBA, M., & Brigitte KOUAME, N. (2026). Antibacterial potential of traditional chewing stick plants Mezoneuron benthamianum and Strychnos afzelii against cariogenic Streptococcus spp. in Côte d’Ivoire. American Journal of PharmTech Research, 16(5), 1-10. https://ajptr.com/articles/AJPTR5160001

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