Development and Validation of Simultaneous UV-Spectrophotometric Method for the Determination of Silybin and Resveratrol in Pharmaceutical (In-House) Gel Formulation

UV-Spectrophotometric method was developed for the simultaneous determination of Silybin (SIL) and Resveratrol (RES) from pharmaceutical (In-house) gel formulation (SIL-RES hydrogel). The in-house SIL-RES hydrogel was formulated by using carbopol as gelling agent. The UV spectrophotometric method involves formation of Q-absorbance equation at 218nm (isoabsorptive point) and at 288 nm, using methanol as a solvent. The linearity for both Silybin and Resveratrol was in the range of 2-12 μg/ml and 1-6 μg/ml respectively. The % recovery was found to be 99-100% for both Silybin and Resveratrol indicating proposed method is accurate and precise for simultaneous estimation of Silybin and Resveratrol in gel.