Burn skin pathogens: Isolation, identification and antimicrobial activity pattern against pyrazole derivatives

Fifty pus samples were collected from burn patients with age ranging 10-55 years with invasive wound infection using sterile cotton swabs. Pure culture were isolated, maintained, preserved and subjected for morphological characterization, accomplished by studying the colony characteristics on different culture plates followed by simple, negative and Gram staining. The cultures then also subjected for biochemical characterization performing various biochemical tests Recovered pathogens were mostly Gram negative. The most common causative agent was found to be Pseudomonas aeruginosa (39.5%), followed by Staphylococcus aureus (25.5%), Acinetobacter baumannii (10%), Klebsiella spp (4.2%), Proteus vulgaris (3.5%) and Escheritia coli (2%). All the previously synthesized compounds were tested on all the isolated pathogens using disc diffusion method and Ciprofloxacin was used as standard drug. The results revealed that out of five, three compounds (1-3) were found to possess significant antimicrobial potential against all pathogens and compounds 4, 5 were also exhibited good activity against E. coli.