In-vitro cytoxicity of M.Maderspantana (Linn.) Cogn. Fruit Methanol Extract against MCF 7 Human Breast Cancer Cell Line and Quantification of Beta Carotenoid in HPLC

The objective of the study was to determine the anticancer efficacy of M. maderaspatana fruit extract against Michigan Cancer Foundation-7 (MCF-7). Different concentration (10, 20, 30, 40, and 50 μg/ml) of methanol extract of M. maderaspatana fruit were used to assess the in vitro cytotoxic effect using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Identification and quantification of beta carotenoid in HPLC. The various concentrations of crude methanol extract (10, 20, 30, 40, and 50 μg/ml concentration) of M. maderaspatana fruit were performed for cytotoxic activity. Effect of inhibition of cell growth showed significantly cytotoxic against Michigan Cancer Foundation-7 (MCF-7)   with an inhibit cell growth by 50% (IC50) of 32 ± 1.0 μg/ml. The βeta- carotene content through spectrophotometry and high performance liquid chromatography HPLC analysis showed that 88ppm of betacarotene present in M.maderspantana fruits.  The results obtained from the study indicate significant cytotoxic activity. The result of anticancer activity study in cell lines of the M.maderspantana extract indicates that has anticancer activity against Michigan Cancer Foundation-7 (MCF-7)   cancer cell lines. The present study concluded that the methanol extract of M.maderspantana possess potent cytotoxic activity.