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American Journal of PharmTech Research

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ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF LIDOCAINE USING RP-HPLC TECHNIQUES

Published in June 2026 Issue 3 (Vol. 16, Issue 3, 2026)

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF LIDOCAINE USING RP-HPLC TECHNIQUES - Issue cover

Abstract

The objective of this work was Analytical Method Development for Determination of Lidocaine Using HPLC methods which are simple, accurate, precise, specific, sensitive, reproducible and economical methods. Forced degradation is carried out to produce representative samples for developing stability-indicating methods for drug substances and drug products. Lidocaine works by inhibiting sodium ion channels in nerve membranes, which prevents the initiation and conduction of nerve impulses, producing local anesthesia. The result for subjected study was found to be Linearity rang (ug/ml) 20-100, Retention time 6.49/ml,% recovery 99%-101%,correlation coefficient (r²)0.9992, Intraday Precision (%RSD) 0.57, Interday Precision (%RSD)0.43. In summary, the study successfully developed and validated a simple, reliable, and stability-indicating HPLC method for the estimation of Lidocaine.

References

  1. [1]1. Shinde R, Chandarana C. “Development and Validation of RP-HPLC Method for Simultaneous Estimation of Lidocaine and Nifedipine in Pure and Combined Dosage Form.” International Journal of Pharmaceutical Chemistry and Analysis. 2023;10(1):1–6.
  2. [2]2. Mashru R, Gandhi M. “Development and Validation of RP-HPLC Method for Estimation of Lidocaine in Various Pharmaceutical Dosage Forms.” International Journal of Research and Review. 2020;7(1):66–72.
  3. [3]3. Kasture A. V., Wadodkar. S.G., Mahadik. K.R., More. H.M. Pharmaceutical Analysis, 23rded., Nirali Prakashan, 2018; 2:18.1-18.11.
  4. [4]4. Indian Pharmacopoeia, “Government of India Ministry of Health and Family Welfare, Published by Indian Pharmacopoeial Commission”. Government of India Ghaziabad, 2018; 3(1):3141-3143.
  5. [5]5. Thapak HK., Sharma J., Boudh B., Banger N., Dwivedia P. Adsorption of copper ions in aqueous media using tea waste and sawdust as an adsorbent. IJIRST Int J Innov Res Sci Technol. 2015;2(3):52-7.
  6. [6]6. Gangola R., Kaushik S., Sharma P. Spectrophotometric Simultaneous Determination of Hydrochlorothiazide and Telmisartan in Combined Dosage Form. Journal of Applied Pharmaceutical Science. 2011 Mar 1; 1(1):46.
  7. [7]7. Skoog D., Holler F., Timothy A and Nieman N (2004) Principle of Instrumental Analysis,5thed, Eastern Press, Banglore, India,2009; 24-29.
  8. [8]8. Pavia DL., Lampman GM., Kriz GS. Introduction to Spectroscopy. Cengage Learning; 2008: 8-14.
  9. [9]9. Anjaneyulu Y., Chandrashekhar K., Manickan V. A. “Textbook of Analytical Chemistry”,
  10. [10]1st ed., Pharma Med Press, Hyderabad, 2008; 253.
  11. [11]10. Douglas A.S., Holler F.J., Crouch S.R., “Principle of Instrumental Analysis”. 6th Ed.
  12. [12] Thomson Publication, 2007; 1-2.
  13. [13]11. Plane JM., Saiz-Lopez A. “UV-visible Differential Optical Absorption Spectroscopy
  14. [14] (DOAS)”. Analytical Techniques for Atmospheric Measurement. 2006; 147-88.
  15. [15]12. Sthal E, Thin Layer Chromatography, “A Laboratory Handbook Springer India”. 2nd ed. 2006: 1114-1123.
  16. [16]13. ICH Q2 (R1), “Validation of Analytical Procedure Harmonized Tripartite Guidline” , 2005.
  17. [17]14. International Conference on Harmonization, Draft Guideline on Validation of Analytical Procedures, Definitions and Terminology, Federal Register, 1995; 11260

Authors (3)

Sandhya S Ahire

Department of P’ceutical Chemi...

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Divyank R. Patil

Reserch Student, KYDSCT, COP, ...

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Sujeetkumar I. Ahire

Department of Pharmaceutics KY...

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Article Information

AJPTR3160004

AJPTR-02-002428

31-41

2026-06-01

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How to Cite

S, S., & R., D. & I., S. (2026). ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR DETERMINATION OF LIDOCAINE USING RP-HPLC TECHNIQUES. American Journal of PharmTech Research, 16(3), 31-41. https://ajptr.com/articles/AJPTR3160004

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