Sandhya S Ahire

The objective of this work was Analytical Method Development for Determination of Lidocaine Using HPLC methods which are simple, accurate, precise, specific, sensitive, reproducible and economical methods. Forced degradation is carried out to produce representative samples for developing stability-indicating methods for drug substances and drug products. Lidocaine works by inhibiting sodium ion channels in nerve membranes, which prevents the initiation and conduction of nerve impulses, producing local anesthesia. The result for subjected study was found to be Linearity rang (ug/ml) 20-100, Retention time 6.49/ml,% recovery 99%-101%,correlation coefficient (r²)0.9992, Intraday Precision (%RSD) 0.57, Interday Precision (%RSD)0.43. In summary, the study successfully developed and validated a simple, reliable, and stability-indicating HPLC method for the estimation of Lidocaine.