ABSTRACT

Teneligliptin hydrobromide hydrate is a new FDA approved drug for treatment of Diabetes Mellitus. Very few methods have been reported for its identified degradation products and their effects on human. A simple, rapid, precise and accurate stability indicating RP-HPLC method was developed and validated for identification of Teneligliptin hydrobromide hydrate and its degradants on Kromacil C18 column using pH 5.5 phosphate buffer and methanol (75:25v/v) as a mobile phase in isocratic mode of elution at a flow rate 1.2 ml/min. The column effluents were monitored by a variable wavelength UV detector at 270 nm. The method was validated as per ICH guidelines. Forced degradation studies of Teneligliptin hydrobromide hydrate were carried out under acidic, basic, neutral, peroxide, photo and thermal conditions. Degradation was observed in basic and neutral stress samples, but not in acid, peroxide, photo and thermal stress samples.

Keyword: Teneligliptin hydrobromide hydrate, RP-HPLC, validation, stability, degradation

ABSTRACT

To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Fosfomycin in Fosfomycin for Injection. The drug is freely soluble in analytical grade water ¹. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics ². It showed absorption maxima were determined in analytical grade water. The drug obeyed the Beer’s law and showed good correlation of concentration with absorption which reflect in linearity ^{4, 5, 6}. The UV spectroscopic method was developed for estimation of Fosfomycin in injection dosage form and also validated as per ICH guidelines. The drug is freely soluble in analytical grade water, slightly soluble in 95% Methanol and Ethanol. Almost insoluble in anhydrous acetone, ether and chlorinated solvent. So, the analytical grade water is used as a diluent in method. The melting point of Fosfomycin was found to be 94 - 95?C (uncorrected). It showed absorption maxima 254 nm in analytical grade water. On the basis of absorption spectrum the working concentration was set on 50µg/ml (PPM). The linearity was observed between 30-70 μg/ml (PPM). The results of analysis were validated by recovery studies. The recovery was found to be 98.75, 101.00 and 99.17% for three levels respectively. The % RSD for precision was found to be 0.41%. A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Fosfomycin in Fosfomycin for Injection dosage form. The method could be considered for the determination of Fosfomycin in quality control laboratories.

Keywords: Fosfomycin, UV Spectrophotometer, Melting Point, Assay Method, Validation, Accuracy, Linearity, Ruggedness, Precision

ABSTRACT

Statins or HMGCoA reductase inhibitors are a group of medicine which effectively prevents cardiovascular disease due to the deposition of atherosclerotic plaques and hence the CV events in elderly population .It also possess inhibitory action on inflammation reaction, improve endothelial function and stabilize coronary plaques. Studies reveals that there is a significant decline in incidence of atherosclerotic CVD and more rigorous study is required to establish association between statin use and reduction in CVD death and all-cause mortality.

Keywords: Cardiovascular Disease, 3-Hydroxymethyl glutaryl Coenzyme A (HMGCoA) reductase inhibitors, Low Density Lipoprotein, Atherosclerosis, Type 2 Diabetes Mellitus.

ABSTRACT

Three groups of rats each consists of five rats were used for this study. Edema was induced by injecting a solution of carrageenan in saline intradermally. Transdermal cellulose acetate phthalate film (with IPM as penetration enhancer) with highest drug release and organogels formulation (Fl: Span 60: 17; Tween 20: 3; Soya bean oil: 40; Drug: 2 and Water: 40) with the highest spreadability, the lowest viscosity and the optimized drug release over 8 h was applied. Results from the in vivo pharmacological study revealed that gel preparation was superior to the patch in reducing the edematous swelling in the rats feet pad over the 1 day of application.

Keywords: Organogels, Solution of carrageenan

ABSTRACT

Black rot has been a main disease constraint of cabbage grown by farmers in Ranchi. Cabbage is popular cultivars of the species (Brassica oleracia) belong to Cruciferae family. The family cruciferae also includes tomato, cauliflower, beans, broccoli, radish, turnip and pepper. In plain area it is grown mainly as a winter crop, whereas in the hilly areas it is grown as a spring and early summer crop. Cabbage is one of the common and most popular winter vegetables grown in India. In Jharkhand the cabbage is grown almost whole year except in the months when temperature rises up to 40-45 degree Celsius. The purpose of this work was to survey, culturing of isolate from cabbage and confirming the severity of Black rot disease in cabbage in the tribal populated area of Kanke block under Ranchi district of Jharkhand state. In morphology and growth characteristics the all the three isolate resemble those describe by Patel et.al.(1949) and Pandey (1980).It is evident from this the isolates of New Zealand and three local isolates of cabbage were morphologically alike being short rod with rounded ends. They are Gram negative and show positive KOH solubility. On the basis of the possession of many phenotypic properties the three cabbage isolates have been tentatively assigned to this pathogen Xanthomonas campestris pv. Campestris (Xcc) .

Keywords : Cabbage, disease, Brassica, Xanthomonas campestris

ABSTRACT

Ficus racemosa Linn. is a moderate-sized avenue tree found throughout India either wild or cultivated for its fruits eaten by villagers. It is popular in Indigenous system of medicine like Ayurveda, Siddha and Homeopathy. Two new triterpenoids of lanostene series were isolated from the roots of Ficus racemosa and their structure were determined as Lanost-20-en-3β-acetate (1) and Lanost-20-en-3β-ol (2)  on the basis of various spectral and chemical studies.

Keywords: Ficus racemosa Linn. Triterpenoids, Lanost-20-en-3β-acetate (1), Lanost-20-en-3β-ol (2)

ABSTRACT

In this study, new Chitosan/4-Hydropxycoumarin films were prepared and characterized. The influence of 4-Hydroxycoumarin on the surface morphology, thermal behavior of the chitosan films were studied. The experimental studies showed that surface morphology becomes uniform and surface roughness increases with increasing the concentration of 4-Hydroxycoumarin in the chitosan film. The appreciable intermolecular interaction among the chitosan and 4-Hydroxycoumarin is confirmed by the FTIR study. The thermal properties of the chitosan films slightly increased with the increasing concentrations of 4-Hydroxycoumarin. Also, the presence of single glass transition temperature in all Chitosan/4-Hydroxycoumarin films suggests the components present in the film were miscible. Due to the existence of 4HC into the chitosan film, the crystalline nature and water contact angle of CS decreases for the C4HC films. It can be expected that, the best properties of Chitosan/4-Hydroxycoumarin films were recorded in the study may play a vital role in food packaging and coating applications.

Keywords: Surface morphology, thermal behaviour, glass transition temperature, miscibility

ABSTRACT

The proton pump inhibitor are rapidly degraded in the acidic medium Thus it has less bioavailability since it is acid labile , mostly available in  the enteric coated tablet to prevent acids degradation .But the onset of action is delayed. It is a great challenge to formulate simple compression solid dosage form of these drugs with considerable stability and efficacy. The Cocrystals of Lansoprazole was prepared by solvent evaporation using nicotinamide as a coformer. The prepared cocrystals were characterized by FTIR, SEM, DSC and XRD study. The cocrystals also evaluated for solubility and acid stability studies. The formation of Lansoprazole cocrystals were confirmed using FTIR, DSC showed the decreased in the melting point of cocrystals corresponds to melting point of pure Lansoprazole. The SEM also confirmed the changes in the habit of crystals. The acid stability study performed using HPLC and confirmed that the Cocrystals of Lansoprazole stable upto 75 % than the pure lansoprazole in 0.1 N HCL.

Keywords: Cocrystals, Lansoprazole, Acid stability, FTIR, Differential Scanning Calorimetry