Evaluation of In-vitro Antimicrobial and Wound Healing Activity of Polyherbal Formulation In Albino Wistar Rats
V. Jhansi Lakshmi*, Gulafashannazreen, E. Lavanya, Amulya .R. Kodukula, Swathi Bagal
Department of Pharmacology,Vignan Institute of Pharmaceutical Technology, Duvvada, Visakhapatnam, Andhra Pradesh, India
In Ayurveda ,individual herbs are not sufficient to get the therapeutic effect in a desired way. Toxicity is reduced and therapeutic effect is produced in a better way when the composition with multiple herbs is formulated in a particular ratio . For developing such an intervention , ethanolic extract of Momordica charantia, Nyctanthes arbortistis ,Azadirachta .indica were used to prepare the poly herbal extract. The zone of inhibition of each bacterial strains (against Bacillus subtilis and Pseudomon asaeruginosa were measured in triplicate .Three Mixtures of extracts with three herbs in different ratio were used for evaluation of antimicrobial effect. Polyherbal mixture 1 Bacillus subtilis (14.8±1.17) Pseudomonas areuginosa(12±1.58) Polyherbal mixture 2 Bacillus subtilis (18±1.19) Pseudomonas aeruginosa (14.2±1.16). Poly herbal mixture3 Bacillus subtilis (25.8±1.11)Pseudomonas areuginosa (22±1.09).Three polyherbal ointments Poly herbal ointmen1, Polyherbal ointment 2,Polyherbal ointment3 were prepared by fusion method . Wound closure of PHO3 have increased (2.83±1.16) when compared to PHO 1(13.68±1.50) and PHO2(5.88±2.40),the PHO promoted the wound healing process through accelerated remodelling process of damaged tissue. Polyherbal ointment where the ethanolic extract was prepared by maceration method and the wound healing is evaluated by excision where it was assessed by rate of wound contraction .Free formulation developed from the combination of ethanolic extracts of azardicta indica ,momodic acharantia , nyctanthesarbortestis showed anti microbial and wound healing effects on various stages on healing cascades. In neem, mainly nimbolide shows anti bacterial activity where in bitter gourd essential obtained from the seed of bitter gourd was tested for anti bacterial activity . In case of wound healing mainly D-mannitol and flavanoid involved. Neem contains active ingredients like nimbi din, nimbin, nimbidole, with anti bacterial, anti- inflammatory that mainly helps in acceleration the wound healing process. Here in Bitter guard , the plant contain cucurbitane, triterpinoids, folic acids glycolipids helpful for wound healing activities. In case of Night flowering Jasmine D-manitol flavanol glycosides, tannic acid , ascorbic acid chemical constituents play role in anti bacterial and anti- inflammatory activity.
Keywords: Momordica charantia, Nyctanthes arbortistis ,Azadirachta .indica, Polyherbal mixture, Polyherbal ointment, Wound closure