ABSTRACT

A new, simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid Chromatography assay method has been developed for estimation of Ornidazole in injection formulations. The separation was achieved by using column Inertsil ODS-3V (150×4.6 mm, 5µ), in mobile phase consisted of acetonitrile and pH 3.0 phosphate buffer, adjusted to pH 3.0 with the help of dilute orthophosphoric acid in the ratio of (10:90, v/v). The flow rate was 2.0 mL/min-1 and the separated Ornidazole was detected using UV detector at the wavelength of 300 nm. Column temperature 25°C and sample temperature ambient and injection volume 20µL. The retention time of Ornidazole, was noted to be 12.05 min respectively. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent.

Keywords: Liquid Chromatography, Ornidazole, Force degradation and Validation.

ABSTRACT

The aim of the current study was to isolate mucilage from five different plants, which was done by maceration, followed by incorporating the extracted mucilage into a tablet formulation and to study its disintegrant property. Comparative results were obtained and varying degree of disintegrant action was observed among the five subject plant sources. It was found that Lepidum sativum seeds showed the highest yield of 12.5%w/w as compared to the remaining four plant products. Additionally, the disintegration time of tablets formulated from mucilage obtained from Lepidum sativum was found to be the least and the tablets disintegrated in 25 seconds. Mucilages are very hydrophilic and are capable of trapping water in their cage-like structures to form a gel. Consequently, when mucilage is mixed with water it swells to many times its original volume as it absorbs water¹. Mucilages find applications in numerous pharmaceutical preparations and perform their role as disintegrants, sustained-release agents, binders, mucoadhesives, to name a few².

Keywords: Mucilage; Paracetamol; disintegrant; swelling ratio; maceration

ABSTRACT

This work is devoted to phytochemical study of tannic extracts from the leaves of Combretum paniculatum (CP), Mallotus oppositifolius (MO) and Ximenia americana (XA), three medicinal plants of Ivorian flora, used in traditional medicine against certain types of cancer. Qualitative analysis by phytochemical screening of these extracts, performed by means of TLC, revealed in addition to hydrolysable and condensed tannins, the presence of phenolic acids and flavonoids. Total tannin contents determined by permanganometry and spectrophotometry are respectively 41.03 ± 0.77% and 12.3648 ± 0.0004% for MO; 20.03 ± 0.44% and 11.7974 ± 0.0001% for XA; 18.76 ± 0.89% and 11.6026 ± 0.0008% for CP. Condensed tannin content in XA leaves (234.135 ± 0.003 µg ECT/mg) is considerably higher than in CP (37.731 ± 0.001 µg ECT/mg) and MO (19.082 ± 0.001 µg ECT/mg). All XA extracts (aqueous and ethyl acetate) have shown good antioxidant activity with CR₅₀s which are lower than that of vitamin C (reference antioxidant). A correlative relationship was found between tannin content and antioxidant activity.

Keywords: medicinal plants, tannins, antioxidant activity, DPPH, Côte d’Ivoire

ABSTRACT

To method develop and validate simple, rapid, cost effective, linear, accurate, precise and economical for the simultaneous estimation of Pregabalin and Methylcobalamin in bulk and tablet dosage form by using UV-Spectrophotometry. The drug obeyed the Beer’s law and showed good correlation of concentration with absorption which reflect in linearity. The UV-Spectroscopic method was developed for estimation of Pregabalin and Methylcobalamin in bulk and tablet dosage form and also validated as per ICH guidelines. The method based on measurement of absorbance at two wavelengths 222 nm (λmax of Pregabalin) and 219 nm (λmax of Methylcobalamin) in Ethanol and distilled water. Linearity graph of Pregabalin and Methylcobalamin were found to be linear in the concentration ranges of 30-150 μg/ml and 0.6-3 μg/ml, respectively, with their correlation coefficient values (R²) 0.999. The low %RSD values indicate method to be accurate and precise. The % recovery of tablets found to be in range of 97-102% and other validation parameter were found to be within the limits as per ICH guidelines.

Keywords: Pregabalin, Methylcobalamin, Simultaneous equation method, UV Spectrophotometer, ICH guideline, Validation.

ABSTRACT

This article provides a review on the pharmacological effects of Aegle marmelos. It is belonging to Rutaceae family. The Aegle marmelos is also known as Bael, Bilwa and wooden apple plant. It contains various bioactive components in leaves, flowers, fruits, wood, root, and bark which has different biological activities and high therapeutic importance. The phytochemical compounds which are isolated from the Aegle marmelos are Coumarins (Marmelosin, marmesin, imperatorin), alkaloids (Aeglin, aegelenine), Tannins (skimmianine), Carotenoids and seed oils and other miscellaneous compounds. The phytochemical components of this plant showed various pharmacological and biological activity against certain chronic diseases like cancer, cardiovascular disease, immunosuppressive disease and gastrointestinal disorder. The different parts of this plant extract possess pharmacological activities like anticonvulsant, antioxidant, anti-inflammatory, antidiabetic, antidiarrheal, antihyperglycemic, anxiolytic, antidepressant, antihistaminic, antimicrobial, hepato protective, analgesic, immuno modulatory, cardio protective and antithyroid activities. The present review summarizes the information about botany, ethnopharmacology, phytochemistry, biological activities and insecticidal properties of A. marmelos plant.

Keywords: Aegle marmelos, botanical description, phytochemical, ethnopharmacology, coumarins, tannins and pharmacological activities^.

ABSTRACT

Syzygium genus contain several plant species which spread across the world, most of them are having diverse biological activity among which some are reported, and some are not. So, Syzygium genus is a subject of interest to researchers. All Syzygium species are actively studied to find new activity or better dose at which they can be used. This review focuses on various species of genus Syzygium, their phytochemical constituents and their pharmacological activities. Syzygium aromaticum (Clove), Syzygium samarangense (Java apple), Syzygium anisatum (Aniseed tree), Syzygium caryophyllatum (Lilly pilly), Syzygium aqeum (Water apple), Syzygium jambos (Mountain Apple), Syzygium cumini (Java plum), Syzygium australe (Brush cherry), Syzygium luehmannii (Riberry) etc., were mentioned in this review article. Biological activities like anti-diabetic, antioxidant, anti-bacterial, anti-inflammatory, platelet inhibition, hepato-protective were found among plants of Syzygium species. Our present review mentions all the activities, anti-diabetic in detail. We tried to gather cumulative data as per our knowledge, hope it is useful to anyone who is studying or researching about Syzygium. Syzygium cumini is given importance in this review article as it has diverse uses and most of the parts of the plant have their own applications in medical field.

Keywords: Syzygium, Anti-diabetic, Syzygium cumini, Myrtaceae.

ABSTRACT

This article provides an overview on the pharmacological effects, uses of various plant parts, Phytochemical constituents of Tulsi. In India, Tulsi is a plant of religious, cultural and medicinal importance from time unknown. It is also known as holy basil belongs to family Lamiaceae. Each part of the plant that is stem, leaves, roots and the whole plant, flowers is known for its medicinal properties. Tulsi has been found to protect organs and tissues against chemical stress from industrial pollutants and heavy metals, and physical stress from prolonged physical exertion, ischemia, physical restraint and exposure to cold and excessive noise. Tulsi has also been shown to counter metabolic stress through normalization of blood glucose, blood pressure and lipid levels, and psychological stress through positive effects on memory and cognitive function and through its anxiolytic and anti-depressant properties. Tulsi its wide pharmacological uses made it a most sought-after plant for scholars and researchers. In this review article we focus mainly on Cultivation, botanical description, taxonomy, medicinal uses, chemical constituents, pharmacological activities of Tulsi like anti-diabetic, hepatoprotective, analgesic activity, anti-inflammatory were discussed in detail. Various species of genus occimum were also mentioned, with their phytochemical constituents and pharmacological activities. This review article contains cumulative information from various research articles.

Keywords: Occimum sanctum, Tulsi, anti-diabetic, anti -inflammatory, analgesic activity.

ABSTRACT

Free radicals are atoms or molecules that have one or more unpaired electrons on its outer orbital, highly reactive, and could damage cell inside human body. Antioxidants are the substances which inhibit oxidation, which have the ability to remove the potentially damaging oxidizing agents in a living organism. Many phytochemicals present in the plants are able to reduce or prevent the oxidative damage to the human cells which can cause even cancer in humans. It is highly vital to know about the antioxidant activities of each plant and the phytocompounds responsible for that. In this study, the DPPH free radical scavenging activity of the extracts of Pomegranate and Citrus (Lemon) is analyzed. And we found Citrus peel extract 2, 2-Diphenyl-1-picryl-hydrazyl (DPPH) assay activity better on comparing Pomegranate peel extract.

Keywords: Herbal Medicinal Plant; Pomegranate; Citrus; DPPH scavenging activity.