P. S. Bedi1*, Ashish K. Sharma2 , Najmeddin, Zayed M Ellali3
1.Department of Chemistry, Wollega University, Nekemte, Ethiopia.
2.Department of Biotechnology, S.B.S.PGI., Balawala, Dehradun, India.
3.Department of Chemistry, Faculty of Science, Agilat, Zawia University, Zawia, Libya.
In present investigation the anticancer properties of Boerhaavia diffusa were studied. Carcinogenicity was induced by DEN i.e. nitrosamine (@ 200 µl/Kg body weight). In a single dose DEN challenged animals were given Boerhaavia diffusa extract (@200mg/Kg body weight) daily and named them as test group. For control group no DEN and no treatment was given. Negative control was given only DEN, to check its carcinogenicity with control and test group. After eight weeks the blood samples were collected to study the effect of carcinogen and plant extract of Boerhaavia diffusa. Various biochemical parameters like : Superoxide dismutase (SOD), Glutathione (GH), Alkaline phosphatase (ALP), Acid phosphatase (ACP), SGOT, SGPT, Triglycerides, Albumin, Catalase (CAT), Lipid per oxidation (MDA), Total proteins (TP), Globulin, Albumin-Globulin ration, Bilirubin, Creatinine, Total cholesterol, HDL, LDL, HDL/LDL ratio and body weight gain were studied. The results of the present study showed that there was a significant decrease in body weight gain among all the groups. Significant increase in SOD, MDA, GH, ALP, ACP, SGPT, SGOT etc. and CAT, TP, Albumin, Globulin, Total Cholesterol, HDL, Triglycerides were significantly decreased as compared with control group. The test group had shown significant hepato protective and anti carcinogenic activity against DEN. The biochemical changes were also supported by histopathological lesions. The present study concludes that plant extract of Boerhaavia diffusa have shown hepato protective and anti liver cancer activity.
Keywords: Anticancer, Hepatoprotective, Diethyl nitrosamine, Boerhaavia diffusa, biochemical parameters and histopathology.